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Vincent Yang. Digestive Diseases.
Phone: 727-5638
Email: vyang@emory.edu
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Spring,Summer,Fall
Lab Positions: 1
Project Description: The focus of our research interest is on understanding the molecular mechanisms that control proliferation and differentiation of the intestinal epithelial cells. In particular, our group has concentrated on the roles played by a number of Kruppel-like transcription factors in regulating these two important biological processes in the gut epithelium. One factor, called Kruppel-like factor 4 or KLF4, is a negative regulator of proliferation that mediates the functions of two important tumor suppressors, APC and p53. The other, called KLF5, is pro-proliferative and mediates the activities of important proto-oncoproteins including RAS and WNT. Our hypothesis that the two KLFs function in the larger network of tumor suppressor genes and oncogenes to regulate intestinal epithelial proliferation and differentiation. The knowledge derived from these studies may impact on the mechanism of gut development and tumorigenesis.
Student Requirements: Molecular Biology, Biochemistry, Genetics
Suggested Reading (References):
(1) Ghaleb, A.M., Nandan, M.O., Chanchevalap, S., Dalton, W.B., Hisamuddin, I. M., and Yang, V.W. (2005) Kruppel-like factors 4 and 5: the yin and yang regulators of cellular proliferation. Cell Research 15, 92-96.
(2) Nandan, M.O., Chanchevalap, S., Dalton, W. B., and Yang, V.W. (2005)Kruppel-like factor 5 promotes mitosis by activating the cyclin B1/Cdc2 complex during oncogenic Ras-mediated transformation. FEBS Letters 579, 4757-4762.
(3) Yoon, H.S., Ghaleb, A.M., Nandan, M.O., Hisamuddin, I.M., Dalton, W.B., and Yang, V.W. (2005) Kruppel-like 4 prevents centrosome amplification following g irradiation-induced DNA damage. Oncogene 24, 4017-4025.
(4) Ouko, L., Ziegler, T. R., Gu, L. H., Eisenberg, L. M., and Yang, V. W. (2004) Wnt11 signaling promotes proliferation, transformation and migration of IEC6 intestinal epithelial cells. Journal of Biological Chemistry 279, 26707-26715.
(5) Hisamuddin, I.M., Wehbi, M., Schmotzer. B., Easley, K., Hylind, L., Giardiello, F.M., and Yang, V.W. (2005) Genetic polymorphisms of flavin monooxygenase 3 in sulindac-induced regression of colorectal adenomas in familial adenomatous polyposis. Cancer Epidemiology Biomarkers & Prevention 14, 2366-2369.
Techniques used in this lab: Cell culture, PCR, Northern and Western blot, immunohistochemistry, transfection, DNA plasmid work
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Linda Gooding. Microbiology and Immunolo.
Phone: 404 7275948
Email: gooding@microbio.emory.edu
Institution: Emory University
Location: On Campus (Emory main campus)
Availability: Summer
Lab Positions: 1
Project Description: We are currently testing a hypothesis that endemic human adenoviruses cause the genomic instability that leads to childhood leukemia. Typical projects would involve testing translocations common to childhood leukemia for their effects on adenovirus replication.
Additional Project Information: We are also developing appropriate culture techniques for primary human mucosal lymphocytes and cord blood
Student Requirements: basic chemistry and biology necessary. Some previous lab work desirable
Suggested Reading (References):
(1) McNees, A., Mahr, J.A., Ornelles, D., and Gooding, L.R. Post-internalization inhibition of adenovirus gene expression and replication in human T cell lines. J. Virol. 78, 6955-6966, 2004
(2) Mahr, J.A., Boss, J., and Gooding, L.R. The adenovirus E3 promoter is sensitive to activation signals in human T cells, J Virol., 77, 1112-1119, 2003
(3) Garnett, C.T., Erdman, D., Xu, W., and Gooding, L.R. Prevalence and quantitation of species C adenovirus DNA in human mucosal lymphocytes. J Virol, 76, 10608-10616, 2002
(4) McNees, A., Garnett, C.T., and Gooding, L.R. The adenovirus E3 RID complex protects some cultured human T and B lymphocytes from Fas-induced apoptosis, J. Virol.76, 9716-9723, 2002
Techniques used in this lab: cell culture, nested PCR, real time PCR, flow cytometry
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Hyunsuk Shim. Winship Cancer Institute.
Phone: 404-778-4564
Email: hyunsuk_shim@emory.org
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Spring,Summer,Fall
Lab Positions: 2
Project Description: The chemokine receptor, CXCR4 is one of critical factors for cancer metastasis by interacting with its ligand, stromal cell derived factor 1 (SDF-1). SDF-1 is expressed in destinations of breast cancer metastasis, including lymph node, lung, liver, and bone marrow. CXCR4 expression is low in normal breast tissues and high in malignant tumors. We developed novel CXCR4 antagonists, which inhibit CXCR4/SDF-1 mediated invasion with high specificity. The long-term outcome that we hope to achieve is the identification of a small molecule that will attenuate tumor metastasis in-vivo while demonstrating a sufficient pharmacokinetic and toxicological profile to merit advancement into human, clinical evaluation. We will also study the specificity or cross-reactivity of our compounds against other chemokine receptors. These potent inhibitors of CXCR4 allow us to study the role of CXCR4 in cancer metastasis and other diseases.
Student Requirements: Biology, Chemistry, Biochemistry
Accepts 2nd year students? Y
Suggested Reading (References):
(1) Liang, Z, Wu, T., Yu, X, Lou, H., Nie, S., and Shim, H. (2004) Inhibition of breast cancer metastasis via CXCR4 antagonists. Cancer Research, 64, 4302-4308.
(2) Liang, Z, Yoon, Y, Votaw, J., Goodman, M., William, L, and Shim, H. (2005) Silencing of CXCR4 blocks breast cancer metastasis. Cancer Research, 65, 967-71.
(3) Koh, A.M., Demiralp, B., Neiva, K., Hooten, J., Nohutcu, R.M., Shim, H., Datta, N.S., Taichman, R.S., McCauley, L.K. (2005) Cells of the osteoclast lineage as mediators of the anabolic actions of parathyroid hormone in bone. Endocrinology, 146(11), 1-13.
(4) Yun, C.C., Sun, H., Wang, D., Rusovici, R., Castleberry, A., Hall, R.A., and Shim, H. (2005) Cellular signaling by LPA2 in colonic epithelial cells is mediated through its interaction with NHERF2. Am. J. Physiology (Cell Physiology), 289 (1), C2-11.
Techniques used in this lab: cloning, cell culture, drug discovery, western blot analysis, HPLC,RT-PCR, etc
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Hui Mao. Radiology.
Phone: (404) 712-0357
Email: hmao@emory.edu
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Spring,Summer,Fall
Lab Positions: 1
Project Description: Developing and characterization of novel MRI contrast agents for target specific cancer imaging in vivo and for other diagnostic imaging applications.
Additional Project Information: Metabolite profiling of biological samples, e.g, tissue, cells, using high resolution solid state NMR.
Student Requirements:
Accepts 1st year students? Y
Accepts 2nd year students? Y
Techniques used in this lab: magnetic resonance imaging, magnetic resonance spectroscopy, medical image process and analysis
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Nandini Dey. hematology/medical oncol..
Phone: 404-778-3037
Email: ndey@emory.edu
Institution: WCI; Emory
Location: On Campus (Emory main campus)
Availability: Summer
Lab Positions: 1
Project Description: Studying the signal-biology in the tumorogenesis of triple negative breast cancer.
Additional Project Information: Studying the role of Ras-P21 signal in the tumorogenesis of glioma.
Student Requirements: Undergraduate Students
Accepts 1st year students? Y
Accepts 2nd year students? Y
Techniques used in this lab: Cell culture, Western blot,DNA purification, Tansient transfection,
Growth curve,confocal and real time video microscopy,Immunofluorescence.
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Pradip De. Hemat & ,Medical Oncol..
Phone: 4047783037
Email: pde@emory.edu
Institution: WCI; Emory
Location: On Campus (Emory main campus)
Availability: Summer
Lab Positions: 1
Project Description: Studying the signal-biology in the tumorogenesis of hormone positive breast cancer.
Additional Project Information: Studying the signal-biology in the tumorogenesis of HER2 over expressed breast cancer.
Student Requirements: Undergraduate
Accepts 1st year students? Y
Accepts 2nd year students? Y
Techniques used in this lab: Cell culture, Integrin directed cell movement, Kinase and GTP-ase assay,Hypoxia, RT-PCR.
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Paul Doetsch. Biochemistry.
Phone: 404-727-0409
Email: medpwd@emory.edu
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Spring,Summer,Fall
Lab Positions: 1
Project Description: Project would address some aspects of the interconnections between DNA repair and DNA damage tolerance systems in the management of DNA damage using a simple eukaryotic model system (yeast) in order to understand this process in higher organisms (i.e. humans) and its relationship to the development of cancer. Techniques would include genetic, biochmical and molecular biological experimental strategies.
Additional Project Information: We are also using the yeast model system and its genetic and biochemical dissectability to elucidate the mechanisms of action of anticancer drugs and to use isogenic strains of yeast as a potential rapid, inexpensive drug screening tool.
Student Requirements: General science background in biology or chemistry. Undergraduate genetics would be very useful but not absolute requirement.. Undergraduate biochemistry would be useful but not required.
Suggested Reading (References):
(1) Evert BA, Salmon TB, Song B, Liu JJ, Siede W, Doetsch PW. (2004) Spontaneous DNA Damage in Saccharomyces cerevisiae Elicits Phenotypic Properties Similar to Cancer Cells. J. Biol. Chem. 279: 22585-22594.
(2) Beljanski V, Marzilli L, Doetsch PW. (2004) DNA Damage Processing Pathways Involved in the Eukaryotic Cellular Response to Anticancer DNA Crosslinking Agents. Mol. Pharm. 65:1496-1506
(3) Doudican NA, Song B, Shadel GS, Doetsch PW. (2005) Oxidative DNA Damage Causes Mitochondrial Genetic Instability in Saccharomyces cerevisiae. Mol. Cell Biol. 25: 5196-5204.
(4) Salmon TB, Evert BA, Song B, Doetsch PW. (2004) Biological Consequences of Oxidative Stress-Induced DNA Damage in Saccharomyces cerevisiae. Nucleic Acids Res. 32: 3712-3723.
(5) O'Rourke T, Doudican NA, Zhang H, Eaton JS, Doetsch PW, Shadel GS. (2005) Differential Involvement of the Related DNA Helicases Piflp and Rrm3p in mt DNA Point Mutagenesis and Stability. Gene 354: 86-92.
Techniques used in this lab: Yeast genetic manipulatiions including strain construction, mutagenesis and recombination assays, and biochemical techniques such as protein purificatiion and Western blot analysis. Cell biological techniques such as fluorescence microscopy and cell sorting and analysis are also likely to be used.
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Chris Yun. Medicine.
Phone: 404-712-2865
Email: ccyun@emory.edu
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Spring,Summer,Fall
Lab Positions: 1
Project Description: Lysophosphatidic acid (LPA) is a lipid mediator with diverse biological properties. The effects of LPA are mediated by the G protein-coupled receptors (GPCR), LPA1, LPA2 and LPA3. We have recently found that LPA2 is over-expressed in several types of cancers, suggesting that LPA2 receptor facilitates tumorigenesis. The aim of this study is to understand the role of LPA2 in colon cancer progression. Student will work on bench learning state of art molecular biologic and physiologic techniques.
Additional Project Information: Mammalian Na+/H+ exchanger NHE3 is the major contributor to Na and fluid homeostasis in intestine and kidney. The aim of this study is to understand the regulation of NHE3 by hormones and growth factors. Student will work on bench learning state of art molecular biological and physiological techniques.
Student Requirements: Biology, Biochemistry, Chemistry.
Accepts 1st year students? Y
Accepts 2nd year students? Y
Suggested Reading (References):
(1) Yun, C. C., Lamprecht, G., Forster, D.V. and Sidor, A. (1998) NHE3 kinase A regulatory protein E3KARP binds the epithelial brush border Na+/H+ exchanger NHE3 and the cytoskeletal protein ezrin J. Biol. Chem. 273, 25856-25863, 1998.
(2) Yun, C.C., Sun, H., Wang,, W., Rusovici, R., Castleberry, A., Hall, R, and Shim, H. (2005) The LPA2 receptor mediates mitogenic signals in human colon cancer cells Am J. Physiol-Cell. 289:C2-C11
(3) Wang, D., Sun, H., Lang, F., and Yun, C.C. (2005) Activation of NHE3 by dexamethasone requires phosphorylation of NHE3 at S663 by SGK1. Am J. Physiol-Cell. 289: C802-810.
Techniques used in this lab: PCR, cloning, Western blot, cell culture
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Dipali Sharma. Winship Cancer Institute.
Phone: 404-778-3265
Email: dsharma@emory.edu
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Spring,Summer,Fall
Lab Positions: 2
Project Description: Selective estrogen receptor modulators, or SERMs, are a class of compounds that can act as estrogen receptor (ER) agonists in some tissues while acting as ER antagonists in others. SERMs are being evaluated and used to treat and prevent such diseases as breast cancer, osteoporosis and cardiovascular disease. The biochemical mechanisms underlying the tissue selectivity of SERMs, however, remain largely unknown. During past couple of years, several laboratories have identified many important co-regulatory molecules that play a central role in mediating the transcriptional activity of many nuclear receptors including ER. Our hypothesis is that the differential expression and interaction of these molecules accounts in part for the tissue-specific selectivity of selective ER modulators, such as tamoxifen and raloxifene.
Also, a major focus of our studies is directed towards understanding, at the cellular and molecular levels, the factors conferring estrogen-independence and antiestrogen resistance and those responsible for affecting estrogen responsiveness. Tamoxifen is the most common endocrine agent used at all stages of breast cancer. ER ? status has been used to identify breast cancer patients who are likely to respond to tamoxifen, but resistance nonetheless occurs in almost 50% of treated ER? positive breast cancer patients. We believe that this resistance could be because of dysregulation of ER interacting co regulatory proteins in breast cancer.
Student Requirements: Cell biology, Molecular biology, Biochemistry
Accepts 1st year students? Y
Accepts 2nd year students? Y
Techniques used in this lab: Cloning, mutagenesis, electrophoresis, transfection, Immunoprecipitaion, ChiP, Methylation Specific PCR, PCR, RT-PCR etc
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Maksym Yezhelyev. Winship Cancer Institute.
Phone: 404-778-5458
Email: myezhel@emory.edu
Institution: Emory University
Location: On Campus (Emory main campus)
Availability: Spring,Summer
Lab Positions: 2
Project Description: Development of Nanoparticle-siRNA Complexes for Anti-Tumor Gene Therapy
Student Requirements: general science course completion, previous lab experience may be helpful but not necessary
Accepts 2nd year students? Y
Suggested Reading (References):
(1) Simultaneous and Quantitative Detection of Multiple Biomarkers in Human Breast Cancers Using Semiconductor Multicolor Quantum Dots
Maksym V. Yezhelyev, Ahmad Al-Hajj, Tonqrui Liu, Ashwan Narayana, Tj Philip, Namita Jayaprakash, Revaz Machaidze and Ruth M. ORegan
Winship Cancer International Seminar, Atlanta, 2006
(2) Optimization of Quantitative Analysis of Multicolor QDs-based Biomolecular Profiling of Tumor.
Ashwan Narayana, Maksym Yezhelyev, Xiaohu Gao, Ahmed Al-Hajj, Tonqrui Liu, Tiji Philip, Shuming Nie, Ruth ORegan
AACR, 2007 Los Angeles, CA
(3) Rapamycin Enhances Cytotoxic Effect of Doxorubicin in Human Hepatocellular Carcinoma Cells.
Yezhelyev, M., Jayaprakash, N., Machaidze, R., Philip, T., Egnatashvili, V., Kooby, D.
Techniques used in this lab: SiRNA transfection, western blotting, immunohistochemistry, cell culturing, cell apoptosis, proliferation and cytotoxic assays
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Neeraj Saxena. Medicine/Digestive Dis..
Phone: 4047275623
Email: nksaxen@emory.edu
Institution: Emory
Location: On Campus (Emory main campus)
Availability: Summer,Fall
Lab Positions: 2
Project Description: Current research project focus on elucidation of the molecular mechanisms underlying links between obesity, adipocytokines and carcinogenesis. Specifically, to understand the suppressive role of Adiponectin in the Leptin induced growth and metastasis of human hepatocellular carcinoma.
Student Requirements: junior or senior with biology background
Accepts 1st year students? Y
Accepts 2nd year students? Y
Suggested Reading (References):
(1) Neeraj K. Saxena, LaTonia Taliaferro Smith, Brandi Brandon, Frank A. Anania and Dipali Sharma (2008). Bidirectional cross talk between leptin and IGF-1 signaling promotes invasion and migration of triple-negative breast cancer cells via transactivation of EGFR. Cancer Research. 2008, (68): 9712-9722.
(2) Neeraj K. Saxena, Paula M. Vertino, Frank A. Anania and Dipali Sharma (2007). Leptin induced growth stimulation of breast cancer cells involves recruitment of histone acetyltransferases and mediator complex to cyclin D1 promoter via activation of Stat3. The Journal of Biological Chemistry. 2007, 282(18): 13316-13325.
8. Neeraj K. Saxena, Paula M. Vertino, Frank A. Anania and Dipali Sharma (2007). Leptin
induced growth stimulation of breast cancer cells involves recruitment of histone acetyltransferases and mediator complex to cyclin D1 promoter via activation of Stat3. The Journal of Biological Chemistry. 2007, 282(18): 13316-13325.
(3) Neeraj K. Saxena, Dipali Sharma, Songbai Lin, Xiaokun Ding, Didier Merlin and Frank A. Anania. (2007). Concomitant activation of the JAK/STAT, PI3K/AKT and ERK signaling is involved in leptin mediated promotion of invasion and migration of hepatocellular carcinoma cells. Cancer Research. 2007, 67(6): 2497-2507.
(4) Songbai S. Lin, Xiaokun Ding, Neeraj K. Saxena, Lance L. Stein, and Frank A. Anania. (2006) Leptin increases Tissue Inhibitor of Metalloproteinase I (TIMP-1) gene expression by a dual Sp1/STAT3 mechanism. Molecular Endocrinology. 2006, 20(12): 3376-3388.
(5) Xiaokun Ding, Neeraj K. Saxena, Songbai lin, Amin Xu, Shanthi Srinivasan, and Frank A. Anania (2005). The role of leptin and adiponectin: a novel paradigm in adipocytokine regulation of liver fibrosis and stellate cell biology. American Journal of Pathology. 2005 June; 166(6): 1655-1669.
Techniques used in this lab: Cell culture, Transfection, Westren-blot, Cloning, plasmid-bacterial work, immunohistochemistry, RT-PCR,
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Gregg Orloff. none.
Phone: 404-727-0308
Email: gorloff@emory.edu
Institution: Emory
Location: Off-campus (but accessible via shuttle, e.g., Grady or VA Hospitals)
Availability: Spring,Summer,Fall
Lab Positions: 2
Project Description: CancerQuest (http://www.cancerquest.org) is an award-winning cancer education project designed to educate and empower cancer patients, caregivers, students and the general public.
We produce content, videos, animations, games, posters and other educational tools.
Students, depending on their interest and skills, could be involved in all aspects of the program including researching, science writing, video creating and editing, graphics, programming, etc.
Student Requirements: Some Biology background and an interest in education/outreach. Computer skills are not necessary but the student must have the desire to learn new programs.
Accepts 1st year students? Y
Accepts 2nd year students? Y
Suggested Reading (References):
(1) Breast Cancer: A Patient's Journey (DVD)
(2) COMPASS: Breast Cancer Edition (DVD)
(3) Gastrostomy Tubes (DVD)
Techniques used in this lab: Science writing, video editing, Flash, HTML (some), Web programming (if interested).
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